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Copure® HPLC Columns Specially for Veterinary Residue

This product utilizes advanced hybrid silica gel and multi-bonded technology, features a broader pH 1-12 and more stable functional groups. The bonded phase is resistant to loss, providing high efficiency and precision in detecting multiple veterinary drug residues. It also exhibits exceptional durability and reliability, enabling continuous testing under strong acidic and basic conditions.

Details

36 Types of Veterinary Drug Residues
Reference Method: GB 31658.17-2021
1. Instrument Conditions
1.1 Chromatographic Conditions
a. Instrument: UPLC-MS/MS (Thermo Fisher TSQ Endura)
Column:Copure®Specially for Veterinary Residue(2.1mm×100mm, 3 μm)
Catalog Number: HSC01
c. Mobile Phase A: Water (0.1% Formic Acid) B:Methanol: Acetonitrile = 2:8 (0.1% Formic Acid)
d. Elution Method: Gradient elution, see Table 1
e. Flow Rate: 0.3 mL/min
f. Column Temperature: 35℃
g. Injection Volume: 5 μL
Elution Program: see Table 1

Time /min	A/%	B/%
0	98	2
3	90	10
8	65	35
10	20	80
11	5	95
12	98	2
14	98	2

Mass Spectrometry Conditions
a: Ion Source: HESI
b: Electrospray Voltage: 3500 V
c: Sheath Gas Pressure: 40 arb
d: Auxiliary Gas Pressure: 2 arb
e: Ion Transfer Tube Temperature: 380°C
f: Auxiliary Gas Temperature: 350°C
g: Qualitative and Quantitative Ion Pairs, Cone
Voltage, and Collision Energy are listed in Table 2
Table 2: Qualitative and Quantitative Ion Pairs, Cone Voltage, and Collision Energy

No.	Compound Name	Retention Time(min)	Parent Ion	Daughter Ions
1	Acetylsulfamethoxazole	3.18	215.0	108.0、155.9*
2	Sulfapyridine	4.67	250.1	155.8*、183.9
3	Sulfadiazine	3.79	251.1	92.1、155.9*
4	Sulfamethoxazole	7.67	254.0	108.1、155.9*
5	Sulfathiazole	4.62	256.0	155.9*、92.1
6	Flumequine	8.59	262.0	201.9、244.1*
7	Oxolinic Acid	10.07	262.0	215.9、244.1*
8	Sulfamethylthiadiazole	5.03	265.1	155.8*、171.8
9	Sulfadimethoxazole	8.12	268.0	113.0、155.8*
10	Sulfamethizole	6.26	271.0	92.1、155.9*
11	Sulfabenzamide	8.59	277.0	107.9、155.9*
12	Sulfadimethoxypyridazine	3.22	279.1	124.0*、185.8
13	Sulfadoxine	5.91	279.1	155.8、185.8*
14	Sulfamonomethoxine	6.21	281.0	155.8*、126.0
15	Sulfamethoxydiazine	6.36	281.0	155.9*、214.9
16	Sulfamethoxypyridazine	7.10	281.0	155.9*、214.9
17	Sulfachlorpyridazine	7.19	285.0	92.1、155.9*
18	Sulfamonomethylpyridazine	7.61	311.1	155.8*、244.9
19	Sulfadiazine	9.05	311.1	155.8*、244.8
20	Sulfabenzthiazole	9.10	315.0	157.9*、159.9
21	Norfloxacin	5.34	320.1	233.0、276.0*
22	Enoxacin	5.15	321.1	234.0、303.0*
23	Ciprofloxacin	5.49	332.0	230.9、288.0*
24	Pefloxacin	5.40	334.2	290.1*、316.1
25	Lomefloxacin	5.67	352.0	265.0*、308.0
26	Delafloxacin	5.76	358.2	314.1、340.0*
27	Enrofloxacin	5.85	360.2	245.0、316.0*
28	Ofloxacin	5.31	362.1	261.1、318.1*
29	Marbofloxacin	4.96	363.1	320.0*、342.0
30	Sarafloxacin	6.28	386.2	299.1、342.1*
31	Difloxacin	6.30	400.2	299.0、356.1*
32	Sulfathiazole	8.11	404.0	148.9、255.8
33	Doxycycline	7.45	445.2	321.0、428.0*
34	Tetracycline	5.55	445.2	410.0*、427.0
35	Oxytetracycline	5.33	461.2	426.0*、443.0
36	Chlortetracycline	6.92	479.1	444.0*、462.0