Copure® HPLC Columns Specially for Synthetic Colorants

11 Types of Synthetic Colorants
Reference Method: GB 5009.35-2023
Column: Copure® HPLC Column for Synthetic Colorants (4.6 mm×250 mm, 5 μm)
Part Number: HDYE01
Mobile Phase: A: 20mmol/L Ammonium Acetate B:
Methanol
Elution Mode: Gradient Elution, see Table 1
Flow Rate: 1.0 mL/min
Column Temperature: 30℃
Injection Volume: 10 μL
Detector: UV Detector
Detection Wavelength: 400-800 nm
Table 1 Elution Program
 

Time /min	A/%	B/%
0.01	90	10
12.0	65	35
19.0	55	45
22.5	50	50
23.0	45	55
24.0	35	65
34.0	35	65
35.0	90	10
42.0	90	10

Liquid Chromatography of 11 Synthetic Colorants

415nm: 1. Tartrazine
2. Quinoline Yellow 1
3. Quinoline Yellow 2
4. Quinoline Yellow 3
5. Quinoline Yellow 4
520nm: 1. New Red
2. Amaranth
3. Carmine
4. Sunset Yellow
5. Allura Red
6. Acid Red
7. Erythrosine
610nm: 1. Indigo Carmine
2. Brilliant Blue 1
3. Brilliant Blue 2
 

Ordering Information

Cat.#	Specification	Qty.
HDYE01	4.6mm×250mm，5μm	1 unit/box