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Biocomma Limited
+86 (0755)-25431879
0086-755-25498726
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  • CommaSil® C4 300 Å HPLC Column
  • CommaSil® C4 300 Å HPLC Column

CommaSil® C4 300 Å HPLC Column

CommaSil®C4 300 Å HPLC Column employs a unique bonding process and fully capping technology to achieve a high degree of coverage of the bonded phase, effectively eliminating the influence of residual silica hydroxyl groups on the analysis. The 300 Å pore size is especially suitable for separating peptide and protein samples, demonstrating strong retention capabilities for both hydrophobic and polar compounds. Additionally, the unique double end-capping technology minimize the impact of residual silica hydroxyls and ensuring excellent peak shapes for the separation of basic and strongly polar compounds. Overall, CommaSil® C4 300 Å HPLC Column is highly suitable for separating peptide and protein samples, maintaining sharp peak shapes while offering excellent selectivity for both hydrophobic and polar compounds.

Details
Determination of Lactoferrin
Chromatographic column: CommaSil® C4 300 Å, (4.6×250 mm, 5um)
Cat. #: HC4536
Flow rate: 1.0 mL/min
Detector: UV detector
Wavelength: 280 nm
Injection volume: 50 μL
Mobile phase: 0.1% trifluoroacetic acid
Table 1. Gradient Elution Program
 
Time (min) CAN (%) 0.1% TFA (%)
0 30 70
5 55 45
10 60 40
12 30 70
16 30 70


 

Ordering Information

Particle size Specification Cat. #
3μm 30×2.1mm HC4114
50×2.1mm HC4214
100×2.1mm HC4314
150×2.1mm HC4414
30×3.0mm HC4124
50×3.0mm HC4224
100×3.0mm HC4324
150×3.0mm HC4424
250×3.0mm HC4524
30×4.6mm HC4134
50×4.6mm HC4234
100×4.6mm HC4334
150×4.6mm HC4434
250×4.6mm HC4534
3.5μm 30×2.1mm HC4115
50×2.1mm HC4215
100×2.1mm HC4315
150×2.1mm HC4415
30×3.0mm HC4125
50×3.0mm HC4225
100×3.0mm HC4325
150×3.0mm HC4425
250×3.0mm HC4525
30×4.6mm HC4135
50×4.6mm HC4235
100×4.6mm HC4335
150×4.6mm HC4435
250×4.6mm HC4535
5μm 30×2.1mm HC4116
50×2.1mm HC4216
100×2.1mm HC4316
150×2.1mm HC4416
30×3.0mm HC4126
50×3.0mm HC4226
100×3.0mm HC4326
150×3.0mm HC4426
250×3.0mm HC4526
30×4.6mm HC4136
50×4.6mm HC4236
100×4.6mm HC4336
150×4.6mm HC4436
250×4.6mm HC4536

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