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  • Modified EC Broth
  • Modified EC Broth

Modified EC Broth

SKU: GF1033F

DESCRIPTION: Modified EC Broth
APPLICATlON: Used for the selective enrichment of Enterohemorrhagic Escherichia coli O157:H7.

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Product Information
PRODUCT NAME Modified EC Broth
PRODUCT CODE GF1033F
DESCRIPTION Modified EC Broth
APPLICATION Used for the selective enrichment of Enterohemorrhagic Escherichia coli O157:H7. 
PREPARATION 1. Weigh 36.62 g of the medium and dissolve in 1000 mL distilled water.
2. Autoclave at 121°C for 15 minutes.
3. After cooling to below 50°C, add filter-sterilized novobiocin solution to achieve a final concentration of 20 mg/L.
COMPOSITION Composition Amount(g/L)
Tryptone 20.0 
Lactose 5.0 
Bile Salt No. 3 1.1 
Dipotassium Hydrogen Phosphate 4.0 
Potassium Dihydrogen Phosphate 1.5 
Sodium Chloride 5.0 
Final pH(25℃) 6.9 ±0.2
STORAGE Store in a dark and dry place at room temperature, and tighten the bottle cap immediately after use.
SHELF LIFE Shelf life of sealed product is 3 years.
PRECAUTIONS 1. NOT for clinical use.
2. Do not use this product if it is lumpy.
3. After use, all contaminated materials must be sterilized or disposed by appropriate procedures.
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