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  • Fluid Thioglycollate Medium
  • Fluid Thioglycollate Medium

Fluid Thioglycollate Medium

SKU: GF1076F

DESCRIPTION: Fluid Thioglycollate Medium
APPLICATlON: Used for the cultivation of aerobic, microaerophilic, and anaerobic bacteria. It is also used for sterility testing of pharmaceutical and biological products, as well as for the anaerobic cultivation of Clostridium perfringens in food.

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Product Information
PRODUCT NAME Fluid Thioglycollate Medium
PRODUCT CODE GF1076F
DESCRIPTION Fluid Thioglycollate Medium
APPLICATION Used for the cultivation of aerobic, microaerophilic, and anaerobic bacteria.  It is also used for sterility testing of pharmaceutical and biological products, as well as for the anaerobic cultivation of Clostridium perfringens in food.
PREPARATION 1. Weigh 29.3 g of the medium and add 1 L of distilled or deionized water. Stir and heat until boiling to ensure complete dissolution. 
2.Dispense the solution into test tubes and autoclave at 121°C for 15 minutes. 
3. Before use, if the pink coloration exceeds one-third of the medium height, heat the medium in a water bath until it becomes colorless. Cool to below 45°C and immediately inoculate with the sample.If the entire tube turns pink, the medium should not be used.
COMPOSITION Composition Amount(g/L)
Tryptone 15
L-Cystine 0.5
Anhydrous Glucose 5
Yeast Extract Powder 5
Sodium Chloride 2.5
Sodium Thioglycollate 0.5
Resazurin 0.001
Agar 0.75
Final pH(25℃)  7.1 ±0.2
STORAGE Store in a dark and dry place at room temperature, and tighten the bottle cap immediately after use. 
SHELF LIFE Shelf life of sealed product is 3 years.
PRECAUTIONS 1. NOT for clinical use.
2. Do not use this product if it is lumpy.
3. After use, all contaminated materials must be sterilized or disposed by appropriate procedures.
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