His Prepacked Columns
biocomma ® CommaX TM His prepacked columns are packed with highly cross-linked Poly(methyl methacrylate) beads which are coated with a propriety hydrophilic layer, then grafted with IDA ligands which donate binding sites to Ni 2+ ions, forming an immobilized metal affinity chromatography (IMAC) medium for purifying recombinant proteins with polyhistidine tags.
Recombinant proteins with polyhistidine tags are retained due to the formation of complex compounds between their polyhistidine tags and immobilized Ni 2+ ions. Impurity proteins without polyhistidine tags are washed off. The target proteins are eluted with an imidazole buffer solution.
CommaX TM His prepacked columns are available in 1 mL and 5 mL sizes.
- Superior biocompatibility
- Uncompromised biological activities
- High purification yields
- Moderate experimental conditions
- Purification of recombinant proteins with polyhistidine tags from various express systems, such as bacterial, insect and mammal cells.
|MPPH01||CommaX TM His Prepacked Columns, 1 mL||5/Box|
|MPPH05||CommaX TM His Prepacked Columns, 5 mL||5/Box|
|ACM050||Ni-IDA Chromatographic Medium, Particle size 50 um, Pore Size 800 A||250 mL/Vial|
Note: CommaX TM His prepacked columns and Ni-IDA chromatographic medium should be stored at 2-8 °C.
- Sample Prep
- Oligo Synthesis
- Nucleic Acid Purification Columns
- Sample Filtration Columns/Plates
- Nucleic Acid Purification Kits
- Protein Purification
- Premix Buffer Powder & Tablets
- Nucleic Acid Purification System
- Lab Filters
- Medical Filters
- Air & Gas Filters
- Water Filters
- Custom Filters & Frits
Consumables & Parts
- Empty SPE Cartridges, Frits & Sorbents
- Empty FPLC Columns
- Empty Affinity Chromatography Columns
- Empty Nucleic Acid Purification Columns
- Empty Synthesis Columns
- Empty Tubes Without Frits
- Empty Column Kits
- Column Accessories
- Autosampler Vials
- Syringe Filters
- Microfiltration Membrane
- Sample Collection Plates
- Electronic Pipettes
- Vacuum Manifolds