His Gravity Flow Columns

His Gravity Flow Columns

Purification of recombinant proteins with polyhistidine tags from various express systems, such as bacterial, insect and mammal cells.
Details

biocomma ® CommaX TM His gravity flow columns are packed with highly cross-linked Poly(methyl methacrylate) beads which are coated with a propriety hydrophilic layer, then grafted with IDA ligands which donate binding sites to Ni 2+ ions, forming an immobilized metal affinity chromatography (IMAC) medium for purifying recombinant proteins with polyhistidine tags.

Recombinant proteins with polyhistidine tags are retained due to the formation of complex compounds between their polyhistidine tags and immobilized Ni 2+ ions. Impurity proteins without polyhistidine tags are washed off. The target proteins are eluted with an imidazole buffer solution.

CommaX TM His gravity flow columns can be operated by gravity flow. They offer higher purification performance if operated by positive pressure via peristaltic pumps or syringes.

Features:

  • Puncturable upper caps confer easy connection with peristaltic pumps and syringes
  • Ease of use, no need for dedicated purification systems

Applications:

  • Purification of recombinant proteins with polyhistidine tags from various express systems, such as bacterial, insect and mammal cells.

Order information:

Cat. # Description Qty.
ACHIS06 CommaX TM His Gravity Flow Columns, 1 mL/6 mL
30/Box
ACHIS06L CommaX TM His Gravity Flow Columns, 3 mL/6 mL, Long-Form 20/Box
ACHIS12 CommaX TM His Gravity Flow Columns, 12 mL 20/Box

Note: CommaX TM His gravity flow columns should be stored at 2-8 °C.

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