CommaPrep™ Plasmid Miniprep Columns
CommaPrep™ Plasmid Miniprep Columns
CommaPrep™ Plasmid Miniprep Columns
CommaPrep™ Plasmid Miniprep Columns

CommaPrep™ Plasmid Miniprep Columns

The columns are based on silica membrane technology and provide fast and reliable extraction of high quality plasmid with a binding capacity of 30 μg.
Details

CommaPrep™ plasmid miniprep columns are supplied with spincolumns and collection tubes.The columns are based on silicamembrane technology and provide fast and reliable extractionof high quality plasmid with a binding capacity of 30 μg.

Extracted plasmid is ready for use in many downstream applications,including restriction digestion, ligation and transformation, PCR,sequencing and library construction.

Features

  • Up to 30 μg high purity plasmid DNA
  • High quality with reproducible yields
  • Single protocol for high or low copy plasmids
  • Suitable for various downstream applications

Typical results

pUC19 was extracted from 3 mL overnight E.coli(DH5a) using columns made by comapy A,company T and Biocomma, and the elution volumewas 80 μL. Meanwhile, extracted plasmid pUC19from 10 mL overnight E.coli (DH5a) using BiocommaHP50, and the elution volume was 100 μL.Then analyze by electrophoresis on a 1% agarose gel.
Lane 1: Column from company A
Lane 2: Column from company T
Lane 3: Biocomma NP30
Lane 4: Biocomma HP50

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Figure 1: Plasmid DNA extracted using various columns




Experimental overview

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Table 1: The yield of plasmid DNA extracted using various columns

A260/280 Concentration (ng/μL) Yield (μg)
Column from company A 1.92 312 25
1.96 300 24
Column from company T 1.86 350 28
1.82 325 26
Biocomma NP30 1.91 350 28
1.88 337 27
Biocomma HP50 2.01 800 80
2.03 850 85

Order information:

Cat. # Description Volume Yield Qty.
NP30 CommaPrep™ Plasmid Miniprep Columns,Capless spin columns, Blue fixing rings 2 mL,800 μL ~30 μg 500/Box
NP30-A CommaPrep™ Plasmid Miniprep Columns,Capped spin columns, Blue fixing rings 2 mL,800 μL ~30 μg 500/Box
NP30-AS CommaPrep™ Plasmid Miniprep Columns,Capped spin columns, White fixing rings 2 mL,750 μL ~30 μg 500/Box
MNP001-1 CommaXP™ Plasmid Miniprep Kit 50 Preps
MNP016-1 CommaXP™ Rapid Plasmid Miniprep Kit 50 Preps

Note: It is recommended that the elution volume should be 60-100 μL by using series of NP30.

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Phone : 0086-755-25431879
Fax : 0086-755-25498726
Email : info@biocomma.com
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