CommaPrep™ Plasmid Miniprep Columns
CommaPrep™ Plasmid Miniprep Columns
CommaPrep™ Plasmid Miniprep Columns
CommaPrep™ Plasmid Miniprep Columns

CommaPrep™ Plasmid Miniprep Columns

The columns are based on silica membrane technology and provide fast and reliable extraction of high quality plasmid with a binding capacity of 30 μg.
Details

CommaPrep™ plasmid miniprep columns are supplied with spin columns and collection tubes.The columns are based on silica membrane technology and provide fast and reliable extraction of high quality plasmid with a binding capacity of 30 μg.

Extracted plasmid is ready for use in many downstream applications, including restriction digestion, ligation and transformation, PCR, sequencing and library construction.

Features

  • Up to 30 μg high purity plasmid DNA
  • High quality with reproducible yields
  • Single protocol for high or low copy plasmids
  • Suitable for various downstream applications

Typical results

pUC19 was extracted from 3 mL overnight E.coli (DH5a) using columns made by comapy A, company T and Biocomma, and the elution volume was 80 μL. Meanwhile, extracted plasmid pUC19 from 10 mL overnight E.coli (DH5a) using Biocomma HP50, and the elution volume was 100 μL. Then analyze by electrophoresis on a 1% agarose gel.
Lane 1: Column from company A
Lane 2: Column from company T
Lane 3: Biocomma NP30
Lane 4: Biocomma HP50

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Figure 1: Plasmid DNA extracted using various columns




Experimental overview

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Table 1: The yield of plasmid DNA extracted using various columns

A260/280 Concentration (ng/μL) Yield (μg)
Column from company A 1.92 312 25
1.96 300 24
Column from company T 1.86 350 28
1.82 325 26
Biocomma NP30 1.91 350 28
1.88 337 27
Biocomma HP50 2.01 800 80
2.03 850 85

Order information:

Cat. # Description Volume Yield Qty.
NP30 CommaPrep™ Plasmid Miniprep Columns,Capless spin columns, Blue fixing rings 2 mL,800 μL ~30 μg 500/Box
NP30-A CommaPrep™ Plasmid Miniprep Columns,Capped spin columns, Blue fixing rings 2 mL,800 μL ~30 μg 500/Box
NP30-AS CommaPrep™ Plasmid Miniprep Columns,Capped spin columns, White fixing rings 2 mL,750 μL ~30 μg 500/Box
MNP001-1 CommaXP™ Plasmid Miniprep Kit 50 Preps
MNP016-1 CommaXP™ Rapid Plasmid Miniprep Kit 50 Preps

Note: It is recommended that the elution volume should be 60-100 μL by using series of NP30.

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    Contacts : Biocomma Limited
    Phone : 0086-755-25431879
    Fax : 0086-755-25498726
    Email : info@biocomma.com
    Website : www.biocomma.com
    Address : Ground FL,Bdg 12, Zhonghaixin Innovative Industrial Park,Ganli Six Rd, Buji St, Longgang Dist,Shenzhen,518114,P.R.China