CommaPrep™ Plasmid Midiprep Columns
CommaPrep™ Plasmid Midiprep Columns
CommaPrep™ Plasmid Midiprep Columns

CommaPrep™ Plasmid Midiprep Columns

The columns are based on silica membrane technology and provide fast and reliable extraction of high quality plasmid with a binding capacity of 100 μg (NP100) or 200 μg (HP200).
Details

CommaPrep™ plasmid midiprep columns are supplied with spin columns and collection tubes.The columns are based on silica membrane technology and provide fast and reliable extraction of high quality plasmid with a binding capacity of 100 μg (NP100) or 200 μg (HP200).

Extracted plasmid is ready for use in many downstream applications, including restriction digestion, ligation and transformation, PCR, sequencing and library construction.

Features

  • Up to 100-200 μg high purity plasmid DNA
  • Simple procedures and easy to operate
  • Single protocol for high or low copy plasmids
  • Suitable for various downstream applications

Typical results

Use NP100 to extract pET32a expression plasmid, and verify with double enzyme digestion.

Lane 1:Plasmid pET32a
Lanes 2-3:Plasmid by restriction digestion
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Figure 1: Plasmid DNA analysed by restriction digestion




Experimental overview

tip3

Order information:

Cat. # Description Volume Yield Qty.
NP100 CommaPrep™ Plasmid Midiprep Columns 15 mL,4 mL ~100 μg 50/Box
HP200 CommaPrep™ High-yield Plasmid Midiprep Columns 15 mL,4 mL ~200 μg 50/Box
MNP001-3 CommaXP™ Plasmid Midiprep Kits 10 Preps

Note: It is recommended that the bacteria culture should be 30-50 mL and the elution volume should be 400-800 μL by using CommaPrepTM plasmid midiprep columns.

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    Contacts : Biocomma Limited
    Phone : 0086-755-25431879
    Fax : 0086-755-25498726
    Email : info@biocomma.com
    Address : Ground FL,Bdg 12, Zhonghaixin Innovative Industrial Park,Ganli Six Rd, Buji St, Longgang Dist,Shenzhen,518114,P.R.China