CommaPrep™ Plasmid Maxiprep Columns
CommaPrep™ Plasmid Maxiprep Columns
CommaPrep™ Plasmid Maxiprep Columns

CommaPrep™ Plasmid Maxiprep Columns

The columns are based on silica membrane technology and provide fast and reliable extraction of high quality plasmid with a binding capacity of 500 μg (NP500) or 1.5 mg (HP1000).
Details

CommaPrep™ plasmid maxiprep columns are supplied with spin columns and collection tubes.The columns are based on silica membrane technology and provide fast and reliable extraction of high quality plasmid with a binding capacity of 500 μg (NP500) or 1.5 mg (HP1000).

Extracted plasmid is ready for use in many downstream applications, including restriction digestion, ligation and transformation, PCR, sequencing and library construction.

Features

  • Up to 500 μg-1.5 mg high purity plasmid DNA
  • Simple procedures and easy to operate
  • Suitable for various downstream applications

Typical results

Table 1: The yield of plasmid DNA extracted using various columns

1 A260/280 Concentration(ng/μL) Yield(μg)
NP500 1.95 300 300
1 1.91 337 320
HP1000 2.01 780 780
1 1.98 800 800

pUC19 was extracted from 120 mL overnight E.coli (DH5a) using columns NP500 HP1000,and the elution volume was 1.0 mL.


Experimental overview

tip3

Order information:

Cat. # Description Volume Yield Qty.
NP500 CommaPrep™ Plasmid Maxiprep Columns 50 mL,22 mL ~500 μg 20/Box
HP1000 CommaPrep™ High-yield Plasmid Maxiprep Columns 50 mL,22 mL ~1.5 mg 20/Box
MNP001-4 CommaXP™ Plasmid Maxiprep Kits 1 1 10 Preps

Note: It is recommended that the bacteria culture should be 100-200 mL and the elution volume should be 1.0-2.0 mL by using CommaPrep™ plasmid maxiprep columns.

    Product Category
    Contact Us
    Contacts : Biocomma Limited
    Phone : 0086-755-25431879
    Fax : 0086-755-25498726
    Email : info@biocomma.com
    Website : www.biocomma.com
    Address : Ground FL,Bdg 12, Zhonghaixin Innovative Industrial Park,Ganli Six Rd, Buji St, Longgang Dist,Shenzhen,518114,P.R.China