CommaPrep™ Plasmid Maxiprep Columns
CommaPrep™ Plasmid Maxiprep Columns
CommaPrep™ Plasmid Maxiprep Columns

CommaPrep™ Plasmid Maxiprep Columns

The columns are based on silica membrane technology and provide fast and reliable extraction of high quality plasmid with a binding capacity of 500 μg (NP500) or 1.5 mg (HP1000).
Details

CommaPrep™ plasmid maxiprep columns are supplied with spin columns andcollection tubes.The columns are based on silica membrane technology andprovide fast and reliable extraction of high quality plasmid with a bindingcapacity of 500 μg (NP500) or 1.5 mg (HP1000).

Extracted plasmid is ready for use in many downstream applications, includingrestriction digestion, ligation and transformation, PCR, sequencing and libraryconstruction.

Features

  • Up to 500 μg-1.5 mg high purity plasmid DNA
  • Simple procedures and easy to operate
  • Suitable for various downstream applications

Typical results

Table 1: The yield of plasmid DNA extracted using various columns

1 A260/280 Concentration(ng/μL) Yield(μg)
NP500 1.95 300 300
1 1.91 337 320
HP1000 2.01 780 780
1 1.98 800 800

pUC19 was extracted from 120 mL overnight E.coli (DH5a) using columns NP500 HP1000,and the elution volume was 1.0 mL.


Experimental overview

tip3

Order information:

Cat. # Description Volume Yield Qty.
NP500 CommaPrep™ Plasmid Maxiprep Columns 50 mL,22 mL ~500 μg 20/Box
HP1000 CommaPrep™ High-yield Plasmid Maxiprep Columns 50 mL,22 mL ~1.5 mg 20/Box
MNP001-4 CommaXP™ Plasmid Maxiprep Kits 1 1 10 Preps

Note: It is recommended that the bacteria culture should be 100-200 mL and the elution volumeshould be 1.0-2.0 mL by using CommaPrep™ plasmid maxiprep columns.

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Phone : 0086-755-25431879
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