RPC Desalting Purification Cartridges
The RPC desalting purification cartridge is packed with monodisperse, microporous PS/DVB resin particles. The highly cross-linked resin is characterized by highly chemical and physical stabilities and can be used in a broader pH range than silica-based packings.
The surface of RPC packing binds DNA molecules via hydrophobic interaction. Then an aqueous solution is used to wash off residual salts. Finally, an organic solvent is used to elute DNA molecules and the eluent is collected in a reservoir.
The RPC desalting purification cartridge has proven to be an effective and economical way to obtain high-purity oligonucleotides.
RPC Desalting purification cartridges can be used for desalting oligonucleotides after synthesis. During the purification process, failure sequences are eliminated and the DMT is subsequently removed, allowing for elution of purified products. Desalting purification cartridges can also be used for desalting oligonucleotides after purification by PAGE or HPLC.
Biocomma provides RPC desalting purification cartridges with various sizes. An appropriate cartridge size can be chosen according to the concentration of oligos. If multiple samples are to be purified, the 96-well plates will be a better choice.
|Cat. #||Name||Purification Scale||Qty.|
|RPC150||RPC Desalting Purification Cartridges, 50mg/1mL||10 nmol-200 nmol||100/PK|
|RPC3100||RPC Desalting Purification Cartridges, 100mg/3mL||10 nmol-600 nmol||50/PK|
|RPC3300||RPC Desalting Purification Cartridges, 300mg/3mL||10 nmol-2.0 μmol||50/PK|
|RPC61000||RPC Desalting Purification Cartridges, 1g/6mL||1.0 μmol-6.0 μmol||30/PK|
|RPC9650||RPC Desalting Purification Plate, 50mg/96well||10 nmol-200 nmol||EA/PK|
- Sample Prep
- Oligo Synthesis & Desalting
- Nucleic Acid Purification Columns
- Sample Filtration Columns/Plates
- Nucleic Acid Purification Kits
- Protein Purification
- Premix Buffer Powder & Tablets
- Sample collectors
- Nucleic Acid Purification System
- Lab Filters
- Medical Filters
- Air & Gas Filters
- Water Filters
- Custom Filters & Frits
Consumables & Parts
- Empty SPE Cartridges, Frits & Sorbents
- Empty FPLC Columns
- Empty Affinity Chromatography Columns
- Filtration Columns
- Empty Synthesis Columns
- Empty Tubes Without Frits
- Empty Column Kits
- Column Accessories
- Autosampler Vials
- Syringe Filters
- Microfiltration Membrane
- Sample Collection Plates
- Electronic Pipettes
- manifolds-vacuum manifolds