Copure® SLE
Copure® SLE
Copure® SLE

Copure® SLE

Novel methodology for liquid-liquid extraction.
Details

Supported Liquid Extraction (SLE) is a very efficient and economical sample preparation methodology. Extracting analytes from aqueous samples is achieved with a simple two-step protocol, i.e., loading and elution.

In SLE, porous diatomaceous earth with high surface area and low chemical activity is packed as stationary support for liquid-liquid partition. While loading a sample, the aqueous sample solution passes through diatomaceous earth beads, allowed to adsorb via capillary action, forming a thin membrane. Then, a small volume of organic solvent percolates and produces an aqueous-organic interface. Because the mass transfer occurs in extreme short paths, analytes can partition very efficiently between the two phases.

Features:

  • High recoveries, more reproducible
  • Simplified protocols, less time-cosuming
  • No need to vigorous shaking, no emulsion formation
  • Using less amount of organic solvents, reducing cost
  • Easy to automation and parallel manipulation

Applications:

SLE is widely used in food safety, environmental protection, clinical diagnosis, forensic science and material inspection. For example:
  • Analysis of drugs and drug metabolites in biological fluids, such as antidepressant sertraline and anti-inflammatory drug ibuprofen
  • Determination of parabens in cosmetics, pharmaceuticals and foodstuffs.

Example: extracting ibuprofen in human serum using Biocomma SLE

Chromatographic conditions:

SLE cartridge: Biocomma SLE, 1 mL

System: Waters Alliance 2690

Column: Welch Ultimate XB-C18 (4.6x250 mm)

Mobile phase: 0.1% formic acid in water : acetonitrile = 20:80 (v/v)

Flowrate: 1 mL/min

Injection volume: 20 uL

Detection: UV at 220 nm

Results show that recoveries greater than 79.7% are achieved in extracting ibuprofen from human serum by using Bicomma SLE (1 mL).

Order information:

Product No. Format Qty.
SLE1CC Copure® SLE cartridges, 1 mL 100/PK
SLE3CC Copure® SLE cartridges, 3 mL 50/PK
SLE6CC Copure® SLE cartridges, 6 mL 30/PK
SLE12CC Copure® SLE cartridges, 12 mL 20/PK
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    Contacts : Biocomma Limited
    Phone : 0086-755-25431879
    Fax : 0086-755-25498726
    Email : info@biocomma.com
    Website : www.biocomma.com
    Address : Ground FL,Bdg 12, Zhonghaixin Innovative Industrial Park,Ganli Six Rd, Buji St, Longgang Dist,Shenzhen,518114,P.R.China